UNFAIR JUSTICE, EVEN AT SCHOOL?

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UNFAIR JUSTICE, EVEN AT SCHOOL?Can aboriginal adolescents be treated fairly, even at school? Why can't some get along? More often than not, aboriginal adolescents struggle to be judged fairly by people of greater authority. They want to try to blend in with their peers and not stand out from the crowd. They want the same respect as most other people and want to be accepted into the society of there outside world. However, they often do not get this necessity by there superiors and are treated unfairly. Dougy and the Fringe Dwellers are two texts that show that these issues occur in the world, even at school.


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Superiors of education should treat each person with the same respect or same identity with each other. Aboriginal adolescence don't feel like that they get the respect that they deserve. Other people claim that if an aboriginal gets in trouble, they won't get as in deep as a 'white' person from the same culture. All the aboriginal adolescence ask is to be accepted and to be treated fairly by there peers. All this does is leave unanswered questions to the outside world. 'Why are they let of the hook? What makes them so special? How come I don't get the same treatment as those people?' If only every single person would be judged fairly by the act that they do and not by the colour of there skin. We live in an unjust world, full of racism and hate, why can't we get along. Aboriginal adolescence deserves as much right as we do. If this problem is solved then many other problems will also be solved. Please note that this sample paper on UNFAIR JUSTICE, EVEN AT SCHOOL? is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on UNFAIR JUSTICE, EVEN AT SCHOOL?, we are here to assist you. Your cheap research papers on UNFAIR JUSTICE, EVEN AT SCHOOL? will be written from scratch, so you do not have to worry about its originality.


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A day at the office

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The clientele that we work with is a rough bunch of people to say the least. About 0% truck drivers, construction and factory workers. Being in South Carolina they are mostly rednecks so I am pretty used to them and the shit they usually bring with them. Until today…. I get this weird child molester type looking fellow this afternoon that I have to give a drug test to. From what I can tell he could be slightly retard or extremely stupid but neither of these traits is unusual. After every instruction I give the idiot (we will call Chester), he busts out some every off the wall comments. Me "Go into the bathroom…" Chester "Are you married? I am!"


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Me "Empty your pockets." Chester "Are you from London? You sound London" (This to me was hilarious because if you don't know me, I have a distinctive southern accent and everyone makes sure I know this. And the language is London?) Me "Are you having a good day?" (I have to make small talk, this keeps me sane.) Chester "I am 4. Do you date older men?" The final one was the kicker. Me "Flip the page over and sign the back" Chester "I would sure like to flip you over." I was shocked. Out of all his incoherent answers I would have never suspected that one. I didn't know what to do. I just started laughing and eventually one of my co-workers had to finish the test. In other events, I did an EKG on a lady with the largest breasts I have ever seen. I confused one as her arm. Both boobs could not fit on the exam table with her at once. They were so large that if I had not gotten someone to help me, when she laid down they seriously would have choked her. This has disturbed me all day long. Please note that this sample paper on A day at the office is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on A day at the office, we are here to assist you. Your cheap college papers on A day at the office will be written from scratch, so you do not have to worry about its originality.


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Discuss the impact of the key related attitudes of job satisfaction and organisational commitment on work performance.

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IntroductionThe concept of "organisation" can conjure a number of images. In order to eliminate any misinterpretation of it's meaning, I will refer to it as being an entitysomething that does not exist in nature but is developed through civilisation. Owing to civilisation, different cultures, norms and social values impact greatly, creating a purposeful and structured common behavioural goal, representing the organisation as being a social unit.In discussing the above statement, I will outline the main significances of attitudes, which affect work performance thus impinging on job satisfaction within an organisation. This essay will target to highlight the realms of an organisation, it's driving forces and both internal and external influences, while also aiming to encapsulate the major theoretical visions, which have brought about the understanding of the behaviour in modern organisations. The immense force of the people within this organisation, is the key to driving it forward.The organisation and it's Driving Force


Organisations, like people, have personalities but the traits, or dimensions, are not necessarily the same because of the differences between organizations and people. While an organization has reporting relationships, people do not. We are surrounded by organisations within every aspect of our daily lives, be it a voluntary organisation, educational, medical, financial; the list is endless. Each and every organisation is distinguishable by the human action within. This human action although it cannot be controlled must be understood in order to gear the efforts of the organisation into goal seeking activities. It is the behaviour of the employers and employees which are the driving force of the organisation and their attitudes, norms and values which ultimately strives for prosperity.The general mantra employees are our most important asset is often repeated. Like many other often quoted sayings this is only partly true. Good employees are invaluable, some may be a liability. The difficulty is what is the difference between good and average employees. In most instances it is their attitude, attitude to work, attitude to customers and attitude to colleagues.A person's attitude to their work is the most important contributor to their productivity, and to the organisation's success. This has been recognised and a number of theories have been suggested to increase motivation. When our values (what we believe is most important) link directly with our behaviour, we become more effective, more productive, and create the life that we want to live. Our work performance improves and leads to a greater sense of job satisfaction and morale within the organisation.Factors that affect work motivation and perception include individual differences, job characteristics, and organizational practices. Individual differences are the personal needs, values, and attitudes, interests and abilities that people bring to their jobs. Job characteristics are the aspects of the position that determine its limitations and challenges. Organizational practices are the rules, human resources policies, managerial practices, and rewards systems of an organization.The purpose of behaviour is to satisfy needs. A need is anything that is required, desired, or useful. A want is a conscious recognition of a need. A need arises when there is a difference in self-concept (the way I see myself) and perception (an individual first impression of any person, place or situation, based on personal instinct).The Theoretical ApproachMcGregor -Theory X & Theory YDouglas McGregor put forward the concept that peoples management behaviour is dependent upon their view of human beings and work. Behind every managerial decision or action are assumptions about human nature (McGregorPeople who support Theory X would believe that the average human being dislikes work and will avoid it if they can and that because of this, people must be coerced to put in the required effort, offered inducements and threatened with punishment. Theory X goes on to hold that the average human being seeks to avoid responsibility, is not ambitious and seeks security before advancement. People who hold to Theory Y, on the other hand, believe that for most people work is as natural as play; that people have capacity for self-control that motivation also arises from the higher order needs such as self-esteem and achievement and that people, if properly managed, will be more than willing to take on responsibility. Finally, theory Y holds that people can be creative and team spirited and that few organisations make use of the abilities that people have. Such beliefs, McGregor said, give rise to very different styles of management - as may seem obvious today. Theory Y may seem very idealistic but McGregor did argue for a situational approach in the application of Theory Y. He argued that one should that take account of the nature of the people, the organisation, the leader him or herself and the social or political environment. One also has to say that there are very many managers alive and kicking today who appear to have a very profound belief in Theory X - to the detriment of many companies and their customers, for not only is it rather assumptive, it undermines the employees within this setting thus retarding their work performance and job satisfaction.Maslow - Hierarchy of NeedsMaslow studied McGregor's and rather than creating such a division as McGregor did, he decided to encapsulate all requirements into what is termed as the "Hierarchy of Needs". This identifies five levels of needs, which are best seen as a hierarchy with the most basic need emerging first and the most sophisticated need last. People move up the hierarchy one level at a time. Gratified needs lose their strength and the next level of needs is activated. As basic or lower-level needs are satisfied, higher-level needs become operative. A satisfied need is not a motivator. The most powerful employee need is the one that has not been satisfied. Abraham Maslow first presented the five-tier hierarchy in 14 to a psychoanalytic society and published it in 154 in Motivation and Personality (New York Harper and Row). Psychological needs are the most basic human needs. They include food, water, and comfort. The organization helps to satisfy employees physiological needs by a salary. Level 1- Physiological needs are the most basic human needs. They include food, water, and comfort. The organization helps to satisfy employees physiological needs by a paycheck. Level - Safety needs are the desires for security and stability, to feel safe from harm. The organization helps to satisfy employees safety needs by benefits. Level - Social needs are the desires for affiliation. They include friendship and belonging. The organization helps to satisfy employees social needs through sports teams, parties, and celebrations. The supervisor can help fulfill social needs by showing direct care and concern for employees. Level 4 - Esteem needs are the desires for self-respect and respect or recognition from others. The organization helps to satisfy employees esteem needs by matching the skills and abilities of the employee to the job. The supervisor can help fulfill esteem needs by showing workers that their work is appreciated. Level 5 - Self-actualization needs are the desires for self-fulfillment and the realization of the individuals full potential. The supervisor can help fulfill self-actualization needs by assigning tasks that challenge employees minds while drawing on their aptitude and training.Herzberg - Hygiene Theory Herzberg describes needs in terms of satisfaction and dissatisfaction. He examined motivation in the light of job content and contest. (See Work an the Nature of Man, Crowell Publications, 166.) Motivating employees is a two-step process. First provide hygienes and then motivators. The fist, motivation, ranges from no satisfaction to satisfaction. The other, hygiene, ranges from dissatisfaction to no dissatisfaction. Satisfaction comes from motivators that are intrinsic or job content, such as achievement, recognition, advancement, responsibility, the work itself, and growth possibilities. Herzberg uses the term motivators for job satisfiers since they involve job content and the satisfaction that results from them. Motivators are considered job enhancers. They are necessary for substantial improvements in work performance and move the employee beyond satisfaction to superior performance. Motivators can be equated to Maslows higher-level needs of esteem and self-actualisation.Dissatisfaction occurs when the following hygiene factors, extrinsic or job context, are not present on the job pay, status, job security, working conditions, company policy, peer relations, and supervision. Herzberg uses the term hygiene for these factors because they are preventive in nature. They will not produce motivation, but they can prevent motivation from occurring. Hygiene factors can be considered job stabilisers because they encourage an employee to stay on a job. Once these factors are provided, they do not necessarily promote motivation, but their absence can create employee dissatisfaction. Hygiene factors correspond to Maslows physiological, safety, and social needs in that they are extrinsic, or peripheral, to the job. They are present in the work environment of job context.Motivation comes from the employees feelings of accomplishment or job content rather than from the environmental factors or job context. Motivators encourage an employee to strive to do his or her best. Job enrichment can be used to meet higher-level needs. To enrich a job, a supervisor can introduce new or more difficult tasks, assign individuals specialized tasks that enable them to become experts, or grant additional authority to employees.One can argue that these theories are rather limited in gaining an employee's true attitude to the job at hand. Firstly, Maslow, researched solely on white middle class America in the 150's, not a broad enough sample to survey and thus rather limited. One can argue that both theorists are absolute in their view as to what one strives for in their employment setting.People's attitudes are learned throughout life. They can be changed especially by a traumatic experience or a period of socialisation. Attitudes are less likely to be changed by training within an organisation. Usually any change programme will result in people saying the right things but not changing their behaviour or attitude. Then everything is forgotten and returns to normal. Again one can trace the limitedness of the above theorists in their research, for they seem to omit to reflect the impact of external forces, which most definitely bear an influence on attitudes within an organisation.The ImpactSo what are these experiences, which bear such an immense impact on work -related attitudes? How are they identified? How are they triggered? More importantly, can they ever be overcome?One major factor affecting work performance is that of stress. The term is often loosely referred to however. "I'm stressed out!" a very common reaction given by one colleague to another. However, it our perception of this reaction which leads to ascertain as to whether or not the colleague in question is under the influence of positive pr negative stress. Put more eloquently, I suppose, there are two separate types of stress, which have frequently never been addresses. One is distress, and can be described as followsWhen employees of an organisation feel stressed, their well being is negatively affected. They dont feel good, and this has a direct affect on the organisation, which, after all, is only as good as its people. The organisational effects of stress can include the following ·High absenteeism and staff turnover ·Interdepartmental conflict ·Deterioration in industrial relations ·Reduction in long-term productivity ·General dissatisfaction, low morale and poor work performance More subtle and even more damaging effects of long term organisational stress include pervasive negativity, lack of organisational commitment, job satisfaction and ultimately organisational breakdown. So, it is in everyones interest that stress is given the recognition that it deserves and policies are developed to tackle it. Any environment can cause stress and some level of stress is just part of everyday life, both within the workplace, in families and in social organisations. However, in order to prevent a situation causing a debilitating level of stress, there must be controls in place. In order to recognise distress in the workplace, a person may behave in one or more of the following ways ·be prone to tearfulness, impulsiveness and out-of-character behaviour ·be aggressive and irritable without apparent cause ·show poor levels of concentration and focus ·develop a variety of illnesses/conditions not medically explained ·exhibit tired, lethargic, apathetic behaviour ·Develop dependencies on alcohol, drugs, people ·Loose interest in many aspects of life within and outside of work. Very severe stress can lead to breakdown, where a person feels so overcome and unable to regain control of life that they need specialist help. There are many ways of preventing such acute situations from developing once the symptoms of stress or potential stressors are identified and treated appropriately. Dont forget, a person might behave in these ways without being stressed, or without the stress being work related, so jumping to conclusions to quickly is not a good idea! The external influences in the employee's life may the creation of such distress and may have nothing whatsoever to do with the prevailing culture within the organisation.The other form of stress is eustress. The former refers to negative stress, where there can be an increase in blood pressure, frequent migraine, often depression as a result of the ability to see the light at the end of the tunnel. The latter refers to postive stress.Eustress can be defined as a pleasant or curative stress. We cant always avoid stress, in fact, sometimes we dont want to. Often, it is controlled stress that gives us our competitive edge in performance related activities like athletics, giving a speech, or acting, reachind a deadline, preparing for an interview etc. If you are involved in an oral interview for a job, you will benefit from a certain amount of stress. It is stress that provides you with focus and gives you your competitive edge that will help you think quickly and clearly and express your thought in ways that will benefit your interview process.Some stressors can cause both good and bad stress. Radiation, left uncontrolled, may cause cancer, and yet, if the radiation is controlled and pinpointed, it may serve to cure some cancers. Exercise is most often a good stressor. But overtraining can cause injury and illness.Reverting to the statement of being stressed out once more!. Research carried out by Wendy Boswell, assistant professor of management in the Lowry Mays College and Graduate School of Business, in the US, on a sample of colleagues within a large financial organisation, highlighted that feeling 'stressed' at work may not necessarily be bad and researchers and practitioners may want to be more discerning as to how stress is regarded and managed in the workplace.Her study revealed that, when employees experienced hindrance-related stress, they were more likely to seriously search for another job with the intent to quit their current one. At the same time, their loyalty to their employer was low. On the other hand, when employees reported challenge-related stress, they were not likely to job search, and actually had low quit intentions. Challenge-related stress also was positively related to organizational loyalty.Which begs the question How can higher stress be related to desirable work outcomes? Boswell says that challenge related job demands may be pressure and anxiety inducing, yet are viewed as rewarding and thus result in enhanced employee attitudes and retention because of the feeling of personal gain that they induce. Also, employees recognize that through these challenges they have the potential for administrative growth, mastery of competencies and personal development. Ultimately, it is the feeling of challenge that indirectly influences the positive work outcomes.In any case, every organisation should include a properly organised programme of activities designed both to satisfy the needs of customers and the marketplace, as well as the needs of the individual employee. We cant smell it, touch it or see it, but stress is a very real danger, which, employees must be protected against under the 18 Safety, Health and Welfare at Work Act. Stress is the invisible disease of the modern organisation and should be addressed within the health and safety system at work in your organisation. Both challenge and hindrance related stress may lead to employee burnout. And while stress may not be totally bad, stress-reduction programs for employees should be aimed at reducing hindrance-related job stress while being careful to not eliminate the more challenge-related experiences in one's job, in particular, those experiences that motivate.Motivation as discussed by Herzberg and Mazlow is used in the workforce not just to attract individuals to that organization but to keep them there. One definition of motivation states that it "is the willingness to do something and is conditioned by this action's ability to satisfy some need for the individual". Robbins(14,p.4) It can be admitted that it a truism that everyone talks about the weather but nobody does anything about it in Ireland anyway! Like the weather, everyone talks about unmotivated employees, clients, or teammates, but unlike the weather, something can be done about lack of motivation. Unmotivated people are viewed as discouraged. Their discouragement may be seen beneath their apathy, absenteeism, uncooperativeness, rebellion, or other symptoms. Discouraged employees need encouragement to become contributing members in the workplace. Reward systems are strategic mechanisms that are used to help achieve the initiator's goals. There are many companies that are beginning to realise the importance of aligning compensation and reward systems aimed at reinforcing the employee's objective.Clearly, a major motivation for working is money for both team andindividual, but money in and of itself is not important, it acquiresimportance as a means of fulfilling needs. The importance of money shouldnot be overestimated. It is clear that money is important if the employee views it as a means to a desired end, but it is definitely not the sole vehicle for satisfying all of the employee's needs (Aldag & Brief, 17). A second area that might motivate individuals and teams is social interaction. Work is social. The importance of the social aspects of work is a function of several factors in addition to the employee's self worth (Aldag & Brief, 15).Employee participation is the extent to which employers move employees to their norms, that is, standards of behaviour, or put more simply the way we do things around here. Employee participation is the extent to which employees share in the decision-making process. It can range from relatively minimal where employers inform employees as to what is occurring in the organization, to relatively extensive, where employees regularly, especially in larger organisations, sit on the board of directors and have influence in major organizational decisions. Often, when people are given input to the decisions that affect them, they are more committed to those decisions so that if employees are given input to the norms, values and behaviours that affect them, they are more likely to be committed to those norms, values and behaviours and thereby be more highly socialized. The extent to which employees are socialised is important to employers because the more socialised they are, the fewer bureaucratic mechanisms are needed to control them.Among the organisational performance outcomes associated with employee commitment are organizational learning; role conflict; autonomy; job satisfaction; willingness to give up; organisational change; and employee involvement. In a work setting, commitment means, at a minimum, that an employee agrees to complete assigned work and meet or exceed specific standards of quality, quantity, and timeliness that add value to the organisation and its results. The level of employee commitment to the job is often the key determinant of whether an employee performs marginally or exceeds expectations.Some examples in gaining employee commitment include setting clear goals for employees that require effort to achieve but are credible measures. Basically, by showing them that there is light at the end of the tunnel, and once achieved, it should be rewarded sufficiently. This can therefore create a work climate where employees feel valued and an integral force in the organisation's mission and not just a mere cog in the machine. Obtaining and maintaining employee commitment can be the most challenging factor of the performance formula. But practicing good performance management techniques offers supervisors and managers the ways and means to sustain and improve that commitment and thereby further leverage their employees capacity to perform.ConclusionThis essay has reviewed the impact of key related attitudes of both job satisfaction and organisational commitment, zoning in particularly on factors such as motivation, stress, conformity and participation. Using the theories of McGergor, Mazlow and Herzberg, I have delineated how their work has influenced organisational behaviour while also highlighting the generalistic aspect of some of their work. Moving forward the best way of obtaining the right attitude amongst employees is by recruiting those people who have the required attitude to start with. Yet, most companies ask for skills and experience in areas where they can easily teach a new employee. Of course in some instances, knowledge and experience are important. But there are less than most people would believe. It may take a couple of months to train someone with no experience. Once they have been trained they will be more productive and outperform experienced people who do not have the right attitude……….! BIBLIOGRAPHYAldag, R. J. & Brief, A. P. (17). Task design and employee motivation.Glenview, IL Scott, Foresman and Company.Campbell, J. P., & Pritchard, R. D. (176). Motivation theory in industrial and organizational psychology. In M. D. Dunnette (Ed.), Handbook of industrial and organizational psychology. Chicago Rand McNally.Maslow, Abraham.154 in Motivation and Personality (New York Harper and Row). Meyer, J. P. & Allen, N. J. (17). Commitment in the Workplace Theory,research, and application. Thousand Oaks, CA SAGE Publications.Morley M., Moore S, Heraty N. and Gunnigle P. (18) Principles of Organisational Behaviour An Irish Text. Gill & McMillan, Dublin http//www.hsa.ie/law/index.htm lhttp//vpr.tamu.edu/advance/stress.html Please note that this sample paper on Discuss the impact of the key related attitudes of job satisfaction and organisational commitment on work performance. is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Discuss the impact of the key related attitudes of job satisfaction and organisational commitment on work performance., we are here to assist you. Your cheap custom college paper on Discuss the impact of the key related attitudes of job satisfaction and organisational commitment on work performance. will be written from scratch, so you do not have to worry about its originality.


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Analysis of the timble

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Arthur Michel TremblayHometown French, Canada (Montreal)Character Bobby StoneHe was a drinker, a office worker, attends church, likes fat woman, who caused this catastrophe. A woman tried to give hem a thimble, but he refused to take it from her, they both caused a scene in the street. She started crying because he would not take her thimble, so a man in the crowd got upset and grabbed Bobby by the collar and threatened him. It was either courage or cowardice, but Bobby hit the man and ran off to work knocking over people who tried to stop him from fleeing. After quitting time at work Bobby sat in his desk thinking of the scene that took place that morning. As he covered and uncovered his eyes the woman was sitting before him, across the desk from where he sat. Once again she begged him to take her thimble, but he refused to do so. She said for the last time I'm going to ask you to take my thimble or I'm going to have to for bid you from having it, and you are going to want to steal it, or kill for it. But he still refused her, so she did it. An just like that he was possessed with the thimble, and he ran after the woman who pretended to flee and lose ground. Bobby assaulted the woman. He kicked her, kneed her, and back slapped her just to get that thimble. He did get it and he was determined to keep anyone else from possessing the thimble. He swore he knew the secret of the thimble now. Before the woman died, she whispered, "In the thimble… In the thimble… I have locked the universe."


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The next morning Bobby Stone awoke he didn't remember nothing . He found the thimble Please note that this sample paper on analysis of the timble is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on analysis of the timble, we are here to assist you. Your cheap research papers on analysis of the timble will be written from scratch, so you do not have to worry about its originality.


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The effect of temperature of the action of the Enzyme Catalase

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AIMEffect of temperature of the action of the Enzyme Catalase.PLANNINGBackground Knowledge


An enzyme is a biological catalyst, it alter the rate of reaction without being changed itself. Enzymes are proteins; they have a very precise three-dimensional shape, which forms a one specific active site on the enzyme. Each enzyme can only convert one kind of substrate molecule in to one kind of product molecule. These are specific.What affects Enzymes?· Temperature- Enzymes stop working if the temperature rises above 40ºC. Increasing the temperature alters the D shape and so the enzyme can no longer fit the substrate.· pH- They work best in neutral conditions neither acidic nor alkaline.What affect does catalase have?Catalase is a very fast reacting enzyme, it is found in many living cells, it breaks down hydrogen peroxide to water and oxygen. In fact one molecule of it can deal with six million molecules of hydrogen peroxide in 1 minute. Hydrogen peroxide is toxic so needs to be changed into harmless substances.CatalaseHydrogen peroxide water + oxygen HO HO + OReferences to practicals referring to enzymes· Biology for You Pg 0 Experiment .1From looking at this I found out that catalase reacts with hydrogen peroxide to give out water and oxygen. Oxygen bubbles produce froth on the surface of the solution. In my forthcoming experiment I will expect to see froth being produced.· Biology- Nelson Science Pg 5 Picture 4From looking at this graph, see below. I have learnt that the affect of temperature does in fact change the rate of reaction. From the graph the reaction reaches 40ºC but then denatures and the rate of the reaction decreases. The rate falls rapidly suggesting denaturing.Taking this information into account I would expect the enzyme catalase to show a similar pattern with respect to the temperature. In order to observe the effect of temperature on catalase we will be maintaining in the amount of oxygen released. The oxygen produces a froth which we will then measure in mm and the volume of oxygen given off which will be measure in cm³Method- measuring the height of froth and volume of oxygen1. Put work shirt on and goggles on. Carry out the rest of safety precautions.. Gather equipment as shown on diagram1.. Using a cork borer make 5 cylinders from the large potato.4. Cut them into all the same length (6cm)5. Using a pestle and mortar mash up each cylinder separately. 6. Measure 5ml of hydrogen peroxide using a measuring cylinder.7. Select the temperature you are going to study0ºC- iced water5ºC-no extra equipment7ºC-water bath required55ºC-water bath required100ºC-beaker of boiling water8. Place on mashed cylinder into a boiling tube add the measured hydrogen peroxide and attach the rubber bung connected to the measuring syringe.. Start stop watch and record volume of gas collected every 0 seconds. At the same time measure the amount of froth produced at 0 seconds intervalsApparatus· 5 beakers· 5 test tubes· Thermometers· Cork borer· Potato· Ruler· Knife· Tile· Measuring syringe · Heat proof mat· Bunsen burner· Tri-pod· Wire gauze· Pestle and mortar· Hydrogen peroxide· Matches· Spills· Ice cubes· Water bath· Goggles · Spatula· Stopwatch· Measuring cylinderFair testIn this investigation I will keep constant the following· The surface area of the potato. I will use the mashed up form as it will be a faster reaction as there is more area to react on, as we have to consider the time span.· The same volume of hydrogen peroxide in each part of the investigation.· The same size equipment e.g. boiling tubes as the readings for the results will be wrong if this is not constant.· Use the same method for each experiment so that there won t be any major differences. Only alter the temperature.· Keep the amount of potato the same amount.· Measure the temperature with a thermometer.AccuracyIn order to make my investigation go to plan I will be as accurate as I can be so I will measure to the correct measuring size.· Measure the volume in cm³ and amount of potato in grams to make sure that they are exactly the same mass before using them in the experiment.· Do the experiment three times to ensure that there isn t an odd result. Three is a good number to use as you can see if there is one odd one where if you just done the experiment twice then you wouldn t know which one odd and which isn t.· Also to average out the results. Safety precautions· Wear goggles· Tuck tie in skirt· Wear work shirt· Handle the hydrogen peroxide with care as it is corrosive and an irritantPredictions and ReasonsFrom my research I think that the enzymes will denature after 40ºC and any other temperature above that. Reason being that enzymes are proteins and their structure is three-dimensional. Increasing the temperature disturbs the intra molecular bonds that hold the D shape. Because of this the shape is altered. Enzymes have an active site. This fits into the substrate molecular (see diagram-lock and key). If the active site is altered the substrate will no longer fit in and so the enzyme doesn t work properly. The rise of reaction rate is also due to the increase in temperature, relating to the kinetic theory. The higher the temperature, the faster they move. This happens but only to an optimum of 40ºC. The curve leading up to the optimum point is gradual but as it is reached it falls dramatically. The reason being that the active site is destroyed therefore no reaction can take place as there is only one specific active site per substrate.OBTAINING EVIDENCEBelow are my table of results which show the height of froth produced in cm and the volume of oxygen in cm³ for each of the three tests at each of the five temperatures studied.TEMPERATURE 10ºCTEST 1 TEST TEST Analysing results and ConclusionFrom my results it appears that catalase works best at 7ºC, and it is virtually denatured at boiling point. Looking at the initial part of the reaction (see graph 1) it is clear that the gradient at the beginning gets steeper when looking at the temperatures between 10ºC-55ºC. At each temperature the line levels off towards the end of five minutes. Looking at graph , there is a steady rise in height of froth up to 7ºC and then a gradual fall up to 100ºC. Looking at my background knowledge and prior experiments using enzymes I can explain my results as follows. Kinetic theory states that particles, which gain heat energy, move more quickly. In our case the reacting particles are the substrate (hydrogen peroxide) and the enzyme catalase. As the temperature is increased the particles of hydrogen peroxide have more energy therefore they collide with the potato more frequently and so increasing the rate at which the product is formed. However at a certain temperature this is no longer the case. This is because enzymes are proteins and proteins can be denatured at high temperatures. This is because proteins have a D shape. In our case the catalase has a certain shape that the substrate fits into. At high temperatures the active site on the enzyme is altered, see diagram below.(Diagram showing active site on the enzyme is altered therefore stopping products being formed)This stops the substrate from 'fittingand so no product is formed. My results do not totally support or undermine my original prediction. The reason being that on graph 1, my results suit my prediction. It shows that the temperature, 7ºC was the fastest and 100ºC is when the enzyme denatures. But in graph , my results undermine my original prediction as at 55ºC the reaction still takes place where as in my prediction I stated that enzymes would denature at 40ºC approximately, I didn t expect this is happen. EvaluationIn my investigation I was pleased with my achievements. In my method, keeping the temperature constant throughout the investigation was hard to maintain, as the temperature of the contents of the tube would change quite quickly and therefore the hydrogen peroxide wouldn t be at the temperature required. To overcome this problem I could keep the test tubes in a hot water bath for all the temperatures making sure that the water bath was the suitable depth. This would ensure constant temperature throughout the whole 5 mins. Also another problem that I encountered was to keep the height of the froth fair. I measured the height of the froth with a 0cm ruler against the test tube rack, with the support of my hand. As I was measuring, my hand would move from time to time and therefore didn t know where I should place my ruler afterwards. To over come this I should attach the ruler onto the test tube rack with cello tape, as it is transparent or maybe use a pointer. With respect to I measured the height of froth in cm, but to be more precise I should have measured it in mm. To over come this I should use a ruler with mm readings. Also another problem that I observed on accuracy was that I didn t allow the temperature to equilibrate to the right temperature. In this case I wasn t using the correct temperature that I wanted, this could have led to some anomalous results. Ideally I should have brought the temperature of the hydrogen peroxide up to the needed temperature before adding to the potato. Looking back at my results I found some anomalous results in my findings. When averaging I used these results, which could of made the average either lower or higher than it should be. To improve this I should have missed these results. Not including some sets of results when making averages may have led to better values. My results are in line with those I predicted. Graphs indicate rise in temperature up a point leads to an increase in oxygen production. This is in line with kinetic theory. However it is very clear that after a certain temperature is reached the enzyme actually virtually stops. This supports my theory of lock and key fit. However optimum activity of enzyme is at about 7ºC this is as we expected. But at 55ºC the enzyme is still not denatured according to my results. This is a higher temperature than I would expect. Possible not allowing solutions to reach temperatures selected has led to an inaccuracy. It may be that in fact that many temperatures of solutions were lower than we stated. Overall, due to reliable repeats and in general predictions being confirmed I feel my results are reliable enough to make a conclusion. The obvious thing I would improve about the measurements I made would be to increase the range of temperatures used. Especially between 55ºC-100ºC. In this way it may be clearer at the temperature which denaturing took place, and would possibly give a graph that resembled the graph in background knowledge. Another way of improving this investigation is to change the method. I measure the volume of oxygen that was produced. In order to get pure oxygen without any other gases that are in the air I would use the same equipment but make sure that the gap between the rubber bung and solution was free from any other gases.AimTo investigate the effect of temperature on the enzyme catalase.PredictionUsing my existing scientific knowledge, I predict that as I raise the temperature to 0, 5, and 40, this is where we will see the greatest reaction. I predict this because enzymes are designed to react best at the body temperatures of the animals to which they belong. For a mammal, this is around 5-6. Catalysts are used to speed up biochemical reactions in the body.An enzyme is a protein molecule that speeds up chemical reactions in all living things. Without enzymes, these reactions would occur too slowly or not at all, and no life would be possible. All living cells make enzymes, but enzymes are not alive. Enzyme molecules function by altering other molecules. Enzymes combine with the altered molecules to form a complex molecular structure in which chemical reactions take place. The enzyme, which remains unchanged, then separates from the product of the reaction. Therefore, an enzyme is a sort of biological catalyst. Those enzymes identified now number more than 700.Enzymes are classified into several broad categories, such as hydrolytic, oxidising, and reducing, depending on the type of reaction they control. Hydrolytic enzymes accelerate reactions in which a substance is broken down into simpler compounds through reaction with water molecules. Oxidising enzymes, known as oxidises, accelerate oxidation reactions; reducing enzymes speed up reduction reactions, in which oxygen is removed.Catalase is present in the peroxisomes (microbody organelles that house various oxidation reactions in which toxic peroxides are generated as side products) of nearly all aerobic cells. It serves to protect the cell from the toxic effects of hydrogen peroxide by catalysing its decomposition into molecular oxygen and water without the production of free radicals (An atom or a group of atoms with an unpaired electron. Radicals are unusually reactive and are capable of causing a wide range of biological damage)Hydrogen Peroxide = HO Hydrogen Peroxide + Catalase = Oxygen + WaterApparatusMeasuring cylinder To hold 100ml of water. This was thoroughly cleaned with tap water beforehand to ensure that the water was not contaminated with anything. This may have led to anomalous results in the long-term.Clay Beehive To provide somewhere to connect the pipe from the mixture to the water. It also acted as a ledge to hold the measuring cylinder as it stood upside-down.Tub To hold water and everything in place.Stopclock To time experiment. I ensured that the experiment was timed as soon as it began because if it hadnt, then the results may have been inaccurate.Water bath To heat chemicals.Conical Flask To hold the yeast and hydrogen peroxide solution together once the experiment had begun. This needed to be cleaned using water to ensure that nothing would contaminate the solution.DiagramMethodI set up the experiment as shown above. I filled the tub full of water. I then placed the beehive in the water. Then I filled a measuring cylinder with exactly 100.0ml of water and placed it on the beehive in the water without letting any of the water in the measuring cylinder escape. I needed it to be exactly 100.0ml so that I could measure it exactly, from a starting point which is relatively easy to remember. After all, 100.0ml is a lot easier to remember than 87.ml! The measuring cylinder was thoroughly cleaned to ensure as little impurities in the water as I could possibly control. Whilst this was being set up, I had already prepared 40.00ml of yeast and 0.00ml of hydrogen peroxide in separate boiling tubes. At this point, it was very important that I kept the two substances apart because if they had been mixed, they would have begun to react The boiling tubes were both cleaned to ensure the chemicals didnt react with anything, and were as pure as possible before the experiment began. When I was certain that it was all prepared, I poured both liquids into a conical flask and fixed on a bung with an attached tube. This operation needed to be practised before the experiment was done for real to ensure it was done as quickly as possible. After all, the hydrogen peroxide and yeast solution will have started reacting as soon as they came into contact. I connected the tube to the clay bee from essaybank.co.uk hive and measuring cylinder, which were both already prepared in the water. From the very beginning, I started the stopclock timing and noted down how much oxygen had been reacted and had travelled down the pipe into the 100ml of water in the measuring cylinder. I noted down the volume of water that was left in the measuring cylinder after five minutes, taking a result every minute. I chose to take down the result for five minutes because any longer than that and there was none, or barely any water remaining in the measuring cylinder. This is because the yeast and the hydrogen peroxide would have finished reacting completely.VariablesVariable The temperatures of the hydrogen peroxide and the yeast.Controlled VariablesVolume of water in the measuring cylinder 100.0mlTimes 0, 1, , , 4, 5 minutesTypes of liquid Water, Hydrogen Peroxide, and Yeast solutionVolumes of substances 40.00ml yeast, 0.00ml hydrogen peroxideRoom temperature 5ºC approximately Temperatures of mixture 0.0?C, 0.0?C, 40.0?C, 50.0?C, and 60.0?C. These must be kept as exactly as possible as yeast is very receptive to changes in temperature.If these variables were altered, it would not be fair test.ResultsGraphs on following pagesConclusionI conclude that my prediction was partly correct. I was correct by saying that the reaction would be quicker as the temperature was 0?, but it was slower at 40? and again quicker at 50?. This may be because the catalyst, in this case catalase worked best at that temperature, allowing for more successful collisions between the yeast and hydrogen peroxide molecules.. I cannot explain these results because I can guarantee that I made sure that it remained a fair test throughout the experiment. I didn t alter any of the other variables. All the other results came out to be what I expected, with the reactions slower at 0? and 60?.EvaluationI was a little surprised at some of the results, and if I were to do this experiment again, I would try to discover what it was that gave these findings. I would also do the experiments for every 5? instead of every 10?. I would also measure other variables, to ensure that there cannot be any more fluke results. I would conduct the experiment more times to get a more accurate average. Although I conducted the experiment as accurately as I could there were many sources of error in the method that I used. Firstly, some help from friends was required to begin the experiment and this lead to a small delay in starting the stopclock. I would have to find a way to be a little more accurate. This would ensure that my results were as accurate and as precise as I could possibly get them.essaybank.coessaybankAim My aim is to find out how temperature affects the breakdown of hydrogen peroxide by the enzyme catalyse. Apparatus & Diagram Delivery tubeMeasuring cylinderBoss & ClampBoiling tube Stand Water basin StopwatchBunsen burnerThermometer Heatproof matWeighing scales TripodBeakerGauzeMeasuring cylinder Safety gogglesScalpel 6 x Boiling tubesPlastic tile PotatoRuler Hydrogen Peroxide Variable to Alter The variable I will alter in my experiment, is the temperature of the catalyse within the potato. This is because; by doing this I will answer my aim and also supply myself with the evidence needed to find out if the temperature of the enzyme (catalyse) does affect the breakdown of hydrogen peroxide. The temperature I measure will be in a regular scale, which starts at 10°c and goes up in tens to 50°c. Variable to keep the same The variables I will keep the same are mainly the amounts of hydrogen peroxide in each of the five boiling tubes, the mass of the potato in each test and that the potato pieces had the same surface area. I have kept these the same, because by doing this I have instantly blocked out any faults in the experiment, which may be due to those variables changing.Safety Used Within the ExperimentThe safety I will have to use is firstly safety goggles through out the experiment and also to have my tie tucked into my shirt, to avoid contact with a naked flame. I will also need a safety tile whilst cutting the potato to ensure that the potato or bench wasn't in any danger of germs. I will also have to consider the fact that other members of the group were to work along side myself, so I will have to be aware of other people's movements. The main term of safety is to be sensible and act quickly if any spillage of Hydrogen Peroxide was to occur. Method To begin with, acquire five boiling tubes and deposit in them ml of Hydrogen Peroxide. Once you placed the liquid in all five boiling tubes, label each one of the boiling tubes starting with 10°c and then ending up with the fifth tube saying 50°c on it. There should still be one empty tube. This boiling tube is used to place the cube of potato in so that it can be heated or cooled to the required temperature, in the water bath. To set up the water bath, you will need a Bunsen burner and the general equipment that is required with it, and a beaker ½ full of water. The water bath for 10°c and 0°c is not filled with water and doesn't require the Bunsen burner; instead, it needs to be ½ full of ice. After you have done this, cut up fifteen pieces of potato 1cm³ and making sure that they have the same properties as each other, for instance the weight, surface area and source of potato must all be the same. Fifteen potato cubes are needed, because the experiment is to be from essaybank.co.uk repeated three times for each temperature, to ensure that I get accurate results and able to draw a average of each temperature. Place one potato piece into the spare boiling tube and according to the label on the tube in which you are going to place the potato in after heating/cooling, put the cube of catalyse (potato) into the water bath for exactly 4 minutes. After the potato has been in the water bath for 4 minutes, carefully take it out of the boiling tube and into the boiling tube in which there is ml of hydrogen peroxide, making sure that the label is correct to the temperature you set in the water bath. The instant you place the potato into the liquid, quickly put the delivery tube that should be placed at one end in a water basin, which leads to a 100 ml measuring cylinder full of water. The water basin should be ½ full of water. The measuring cylinder would collect all the hydrogen being produced. Let the measuring cylinder collect the hydrogen for 4 minutes and the record the amount of water displaced. Repeat this procedure for each temperature three times to insure we have an average amount of hydrogen produced. Prediction By looking at my experiment, I predict that the rate of break down of hydrogen peroxide by the enzyme catalyse will increase as the temperature increases, but I also know that this will only increase to a certain point. This point is the denature stage, which usually occurs around 50°c 60°c. So, I will be expecting to see results that increase and the suddenly decrease. ExplanationI have predicted the above because from my knowledge, I have studied that in general terms enzyme activity increases as the temperature increases. This is because the molecules have more energy and therefore collide more often. This happens to a point where the enzyme becomes denatured and no longer works as usual. This is all because of how enzymes work, which is shown in the diagram below. EnzymeSubstrate This is how the enzyme looks before heating. Notice how the substrate fits exactly in the active site. Enzyme Substrate This is how the enzyme looks after heating of 50°c-60°c. The substrate no longer fits the active site.This sketch graph below shows how the activity of an enzyme increases as the temperature goes up, and it also shows how it suddenly drops as the enzyme is denatured. Preliminary Work I did a brief experiment in order to receive a more knowledgeable view over the final task. The changes that had to be enforced were that the hydrogen peroxide, which I had as ml in the method, had to be increased to 4 ml per boiling tube. This is because the 1cm³ potato didn't fully get coated with the hydrogen peroxide. The usefulness of the preliminary work was that I got the idea of using instruments like a measuring cylinder for example, with more accuracy by measuring below the meniscus. The main advantage of the preliminary experiment, was that in my method I stated that one should use a 100 ml measuring cylinder for the retrieval of oxygen, but this test proved to me that the results were between 0-10 ml. 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