Osmosis

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Osmosis in Potato CellsAimIn this investigation my aim is to explore the effect of varying the concentration of a certain sugar solution on the amount of osmotic movement there is between the solution and the size of the potato piece used.Hypothesis


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Osmosis is defined as the passage of water molecules moving from a region of low concentrated solution into a region of highly concentrated solution. This course of action takes part through a partially permeable membrane, such as a cell membrane that lets minor molecules such as water through but does not allow bigger molecules to pass through. This type of diffusion and is a passive process, which does not require any input of energy. In this case, the tiny holes in the membrane of the potatoes will allow the water molecules to pass through in and out of the solution and potato, depending on the concentration gradient of the two substances. Water molecules voyage in both directions but there is a net movement of molecules from a higher to lower water potential. So, when the water concentration is inferior in the tissue, the water will go inside the tissue of the potato, and the potato will gain weight. And if there is very little difference in the water concentrations, there shouldn't be a colossal change in weight and if there is a higher concentration of water in the potato, the water will go out of the potato. The molecules will continue to diffuse until the area in which the molecules are found reaches a position of equilibrium, meaning that the molecules are aimlessly dispersed throughout an object, with no area having a higher or lower concentration than any other. Water can move from A to B and B to A. though, more water passes from A to B because water is more expected to hit the membrane since there is higher water potential in A.PlanI apparatus I need will be oMeasuring Cylinder to measure the solutionsoDistilled Water part of the experimentoSucrose Solution part of the experimentoPotato part of the experimentoTissue Paper to dry the potato chipsoElectronic Balance to weigh the potato chipsoCore Borer to cut out potato piecesoTest Tubes for the experiment to take place inoBeakers to make the different concentrations of solutionThe apparatus will be set us as shown belowI will make sure that I precisely measure the mass of the potato pieces before and after incubation by making sure that I have dried them thoroughly by taking away all excess fluids that could influence the mass. This will be done so that I can work out which solution osmosis has taken place in the most.I will change the concentrations of the sucrose solution using a range of concentrations. I will use at least five concentrations that will be very equally spread out. The concentrations I will be using are 0%, 0%, 40%, 60%, 80% and 100% of sugar solution. Other factors involved which might affect my results. These areoTemperature of wateroSize of chipoLightoMass of potatooVolume of solution potato is in oType of potatooTime left in solutionoSurface area of potato chipoUsing the same balance to measure chip If I wish to make this a fair test, then I will have to think about these other factors. Doing the tests at one permanent temperature will control the temperature of the solution. For the principle of my experiment I am going to do all of them at room temperature. If the temperature is too high then osmosis will take place more repeatedly as the molecules will be more energetic, whereas in a low temperature the molecules will be less active.The size of the potato is very significant. This is because if the size of the potato varies, so will the amount of mass it can loose or hold. I'm going to use a core borer in order for the sizes of the potato chips to be the same shape and size.The light moderation can't actually be measured or kept the same, as the pieces will be moved from one room to another for storage. Yet, I will try to keep the pieces in the same position whilst in storage.The mass of the potato is a variable, and this means that it will be measured all the way through the experiment. I will measure the mass in grams. The potato piece will be measured before it is put in the solution, and afterwards. This will allow me to see whether osmosis has taken place, and how much osmosis has occurred.The volume of the solution that the potato pieces will be kept in must be the same. The piece must be totally enclosed in the solution because if it isn't the result of osmosis might not occur to it's fullest. The quantity of solution will be kept the same because all the pieces are the similar sizes. The amount of solution I will cover each piece with will be 50ml.While using the balance, I will make sure that it is reading zero before I weigh each potato so that I don't get any false readings. To make the mass readings a lot fairer, I will take each piece; roll it gently on a paper towel, to remove all extra solution whilst not squeezing the potato piece. I'm also going to use the same balance to weigh my chips. This is because the measurements may vary slightly between scales. The type of potatoes I will use is going to be kept the identical, because different potatoes may soak up at unlike rates. For this experiment I am going to use the same potato because every potato has somewhat different cells. The period the potato piece is left in the solution must be kept the same for each piece. This is since more or less solution may be engrossed depending on time. I am going to keep each potato piece in each solution over night for 4 hours. The surface area of the potato pieces will be kept the same by having all the pieces the same size. This must be kept the same because the amount of surface area exposed to the solution may affect the rate of osmosis. The thickness of the chip will already be the same due to the size of the core borer.After the experiment, I will dry each potato piece and weigh each of them in the same way as before. When taking readings from the balance I will take them to decimal points.Small things such as grubby test tubes, and slightly fractured measuring cylinders could affect the results, and therefore I will have to think about these accounts. In order to make sure that my results are precise I will repeat every concentration at least once. This is so that if any of my concentrations go wrong then it can be simply seen. Thus, this will also make my results more reliable.SafetyIn every experiment safety is a vital aspect, even if the experiment seems to be very harmless. That is why we should take all precautions into consideration.I will be using a core borer, which could easier harm someone if not handled appropriately. I will also try to be careful when handling the solutions, as not to get any within our bodies, as we are not fully aware of the harm that it could do to us.Other than that, there aren't any major matters to fret about.MethodI will firstly set up the equipment as shown in the diagram above. I will then get my pieces of potato ready. I will push the core borer through the potato, cutting out 1 evenly sized potato pieces. I will then dry them out using tissue paper. After they are dry, I will weigh each potato piece exact to decimal places and then record the results onto a table. I will then put one of each potato piece into each of the tube tubes and then make up the 6 solutions as shown in the table belowrequired concentration of sugar solution (%)volume of sugar solution used (ml)volume of distilled water used (ml)final volume of solution (ml)0000041604081060180801640100000I will then place each solution in individually labelled test tubes, but only 50ml of each. I will make 100ml of each solution, as it will then be fair when I repeat my tests. After I have added each solution in test tubes I will cover them using a cork. This is so that all of the essential gases don't escape from or interrupt with the experiment. They will then be left over night, for 4 hours. The next day I will take each of them out of the test tubes dry them and then weigh them once more to find out how much they have grown or shrunk. This will then help me to discover how much osmosis has taken place within each potato piece.PredictionFor this investigation I think that the lower the concentration of the sugar solution in the test tube the larger the mass of the potato will be. This is because the water molecules pass from a high concentration, (i.e. in the water itself, to a low concentration, i.e. in the potato piece). Therefore, the pieces in high water concentrations will have a larger mass than in higher sugar concentrations. When plant cells are placed in concentrated sugar solutions they lose water by osmosis and they become flaccid. This is the precise opposite of turgid. The contents of the potato cells shrink and pull away from the cell wall. These cells are thought to be plasmolysed. When plant cells are located in a solution, which has exactly the same osmotic strength as the cells they are in a state between turgidity and flaccidity. We call this incipient plasmolysis.Plants cells can exist in three forms1.A Fully Turgid CellI think that the cell will become fully turgid in a very feeble sugar solution or pure water, therefore, in the 0% concentrated solution. This is because the cells have gained water and so its volume increases. .A Turgid CellThis is how a typical cell looks. I think the cell will be like this between the 40% and 60% concentrated solutions. This is since it is in a weak in the sugar solution or has the same concentration as the plant cell. .A Plasmolysed CellI think the cell will become like this in a very strong sugar solution such as 100%. This is because the cell has water and so its volume has reduced causing it to shrink and wither. ResultsoExperiment Oneconcentrationinitial massafter masschange in mass% increase04.04.80.800%0.74.8-0.-4%404.0.46-1.57-%604.07.0-.05-50%804.15.05-.10-51%1004.1.84-.-57%oExperiment Twoconcentrationinitial massafter masschange in mass% increase04.05.010.811%0.70.65-1.05-8%404.5.4-1.8-4%604.18.16-.0-48%804.07.07-.00-4%1004.401.81-.5-5%oAverageconcentrationinitial massafter masschange in mass% increase04.154.50.810%0.7.74-0.-6%404.14.45-1.70-41%604.1.0-.04-4%804.11.06-.05-50%1004.1.8-.4-58%Analysis From my graph I can see that the concentration of solution already inside the vacuole of the piece of potato that I had used would have about - 10 % sucrose concentration. I am able to find out this information by looking at my graph and following the 0% along the x-axis and then when I met up with my percentage line then I had drawn I went down and found out the value. ConclusionIn this experiment, I believe that I have composed enough data to support my prediction. This investigation was somewhat successful meaning my results collaborated my prediction.From my experiment and the graph, I can see that there is a relationship between the concentrations of the sucrose solution and the percentage increase in mass of each potato piece. As the concentration of the sucrose solution increases the percentage increase of each potato piece decreases in weight. Therefore, there is a pattern in this experiment. This is because if there is a low concentration of sucrose in the solution then the mass will increase more than if there was more sucrose in the solution. This shows that the percentage increase in the mass depends on the concentration of the solution. If the concentration is low then the percentage in mass is higher.My graphs and results show thatoOsmosis actually took place in the experimentoAs the molars increased, the percentage of the weight difference decreasedThere doesn't seem to be any results, which demoralize my predictions, and I didn't seem to have any anomalous results, which meant I didn't have to repeat this experiment more than twice.This experiment helped me find out that osmosis between liquid substances with a partially permeable membrane, and that the higher the water concentration is, the larger increase there is in grams. EvaluationThe experiment did go as I had expected although I think that there were a few anomalous results. Therefore, this means that many factors could have gone wrong in the process of and after doing my experiment. These are that I may have unintentionally used dissimilar potatoes whilst cutting my potato pieces and I may not have mixed my solutions properly as my calculations may have been wrong and so this would then lead to incorrect measurements. Also, the potato pieces may not have been dried properly which would make the test inequitable and whilst weighing the potato pieces the balance may not be set to exactly 0, which would have an effect on my results. If I were to do this experiment again I would definitely repeat the experiment at least once again as this would help me to see what may have gone wrong with my anomalous results. I do not think that my results are very reliable as I do not have a very good line of best fit because a few points on the graph are not so close to the line of best fit. Also, my repeats are very comparable for some concentrations but they are not so similar for other concentrations, which could be because whilst making my solutions the measurements were not done appropriately. Therefore, I would say that my irregular results do cast some sort of doubt on my conclusion, as my line on best fit does fit quite a few of the points on my graph. Other experiments which could have be done to find out more about this subject would be to see what different types of potatoes could be used, or a range of different surface areas could be exposed to the solution or the temperature in which the potato pieces are stored. Nevertheless, the other factors must be kept the same. These experiments would also help to show which environment osmosis takes place best in and how each factor affects it. Please note that this sample paper on Osmosis is for your review only. 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