Enzyme Lab

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Discussion1.Baseline value for HO = |(final burette reading initial reading)| = |(6.mL 10mL)| = |-.1mL|


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= .1mL By subtracting the initial reading from the final burette reading and then taking the absolute value of the value, you would get the baseline value, which is .1mL.The percent of HO that spontaneously decomposes in 4 hours = mL baseline mL 4 hours X 100mL baseline= 4.1mL .4mL X 1004.1mL= .7mL X 100 4.1mL= 70% 4.1mL= 17.1%Thus, the percent of H O that spontaneously decomposes in 4 hours is 17.1%..The amount of HO used for each reaction time = (Baseline value amount of KMnO4 used)SampleClass Average 10 seconds = .1 .5 = 0.610 seconds = .1 .7 = 0.40 seconds = .1 .0 = 1.10 seconds = .1 .5 = 0.660 seconds = .1 1.7 = 1.460 seconds = .1 1.7 = 1.410 seconds = .1 1.4 = 1.710 seconds = .1 1. = 1.180 seconds = .1 1. = 1.8180 seconds = .1 .6 = 0.54.Refer to the graph at end of lab writeup5.The rate of the reaction = difference between values difference between times0 10 seconds = 0 0.6mL = - 0.6mL = 0.06mL/seconds 0 10 sec- 10 sec10 0 seconds = 0.6 1.1 mL = - 0.5mL = 0.05mL/seconds10 0 sec - 0 sec0 60 seconds = 1.1 1.4mL = - 0.6mL = 0.0mL/seconds 0 60 sec - 0 sec60 10 seconds = 1.4 1.7mL = - 0.mL = 0.005mL/seconds 60 10 sec - 60 sec10 180 seconds = 1.7 1.8mL = - 0.1mL = 0.0017mL/seconds10 180 sec - 60 sec 6.The rate of the enzyme reaction is the highest in the beginning or between the ten to 60 second intervals of the reaction. This is because the catalase did not have enough time to break down the enzyme and slow down its reaction.7.The rate of the enzyme reaction is the slowest in the end or between the 60 and 180 second intervals. This is because the hydrogen had a lot of time break down the enzyme and slow it down, thus slowing down the reaction its reaction. 8.The inhibiting effect of sulfuric acid on the function of catalase is that sulfuric acid stops the function of the catalase. This is because the sulfuric acid lowers the pH, denatures the enzyme, and as a result stops the enzyme's catalytic activity. So, the sulfuric acid affects the function of the catalase by not letting it function at all. Please note that this sample paper on Enzyme Lab is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Enzyme Lab, we are here to assist you. Your persuasive essay on Enzyme Lab will be written from scratch, so you do not have to worry about its originality.


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